Projects

Individual, independently funded, projects that contribute to the goals of the Human Glycome Project are:

1) Diversity of the human glycome

Short Name	Description	Coordinating Institution
Determining the human glycome	To isolate and structurally characterize all glycans in the human body	Harvard Medical School (Richard Cummings, rcummin1_at_bidmc.harvard.edu)
Determine developmental dynamics in the human glycome	Use pluripotent stem cells (healthy and diseased), differentiate into derivatives of all germ layers and correlate the glycome with state of differentiation.	Hannover Medical School (Falk Buettner, Buettner.Falk_at_mh-hannover.de)

2) Inter-individual variability of the human body fluid glycome

Short Name	Description	Coordinating Institution
Variability of the plasma glycome in health and disease	To study relevance of inter-individual variability of either total plasma N-glycome, or IgG glycome in 100,000 individuals	Genos Glycoscience Research Laboratory (Gordan Lauc, glauc_at_genos.hr)
Total plasma and IgG glycomes, statin therapy, and ASCVD events	To determine predictive power of total plasma and IgG glycome for atherosclerotic cardiovascular disease	Harvard Medical School (Samia Mora, smora_at_bwh.harvard.edu)
Longitudinal changes in the human glycome	To profile total plasma and IgG glycomes in 2000 individuals in three different time points	Kings College London (Frances Williams, frances.williams_at_kcl.ac.uk)
Glycome and microbiome interactions	To evaluate interactions between human glycome and microbiome at multiple levels	Kings College London (Tim Spector, tim.spector_at_kcl.ac.uk)
Glycomics in The Perth Longitudinal Study of Ageing Women	Using the glycome to stratify longevity, cardiovascular disease risk and mortality in women aged over 70 years	Edith Cowan University (Wei Wang, wei.wang_at_ecu.edu.au)

3) Inter-individual variability of the human tissue glycome

Short Name	Description	Coordinating Institution
Tissue-specific glycomics	To determine the organ-specific tissue glycosylation features in health and disease	Institute for Glycomics, Griffith University (Daniel Kolarich, d.kolarich_at_griffith.edu.au)
Human Gut glycobiome	The analysis of host mucins and the interplay with prebiotics and microbial cell surface polysaccharides/biofilms	University of Manchester (Sabine Flitsch, Sabine.Flitsch_at_manchester.ac.uk)
Determine the glycolipidome in kidney and heart	Isolate and structurally characterize kidney glyco(sphingo)lipids as a function of age and disease	Hannover Medical School (Falk Buettner, Buettner.Falk_at_mh-hannover.de)
Variability in human milk oligosaccharide (HMO) composition	To study fixed and modifiable maternal factors that influence inter- and intraindividual variation human milk oligosaccharide composition	UC San Diego Larsson- Rosenquist Foundation Mother- Milk-Infant Center of Research Excellence (Lars Bode)

4) Functional relevance and the regulation of the human glycome

Short Name	Description	Coordinating Institution
Genetic regulation of protein glycosylation	To identify gene network that regulate IgG and plasma protein glycosylation by performing genome wide association studies	Genos Glycoscience Research Laboratory (Gordan Lauc, glauc_at_genos.hr)
Glycomic signatures of human diseases	To determine changes in the glycome that associate with major human diseases	Leiden University Medical Centre (Manfred Wuhrer, m.wuhrer_at_lumc.nl)
Cancer glycomics	Determining the glycosylation signatures of human cancers	Institute for Glycomics, Griffith University (Daniel Kolarich, d.kolarich_at_griffith.edu.au)
Host glycomic signature of HIV infection	Investigating the role of host glycosylation machinery in HIV persistence and immunopathogenesis	The Wistar Institute (Mohamed Abdel-Mohsen, mmohsen_at_wistar.org)
Total plasma and IgG glycomes, statin therapy, and ASCVD events	To determine predictive power of total plasma and IgG glycome for atherosclerotic cardiovascular disease	Harvard Medical School (Samia Mora, smora_at_bwh.harvard.edu)
Glycomics and glycoproteomics in liver disease	To analyse tissue and biofluid glycans and glycopeptides as biomarkers of liver disease	Medical University of South Carolina (Anand Mehta, mehtaa_at_musc.edu)
Urine glycomics and glycoproteomics	To analyse tissue and biofluid glycans and glycopeptides as biomarkers of prostate disease	Medical University of South Carolina (Richard Drake, draker_at_musc.edu)
Plasma, tissue, urine and gut bacteria glycome in cancer, neuron, and diabetes	To characterize N-glycan, O-glycan and proteoglycan in serum, urine and intestinal bacteria glycome in individuals	Shanghai Institute of Materia Medica, Chinese Academy of Sciences (Kan Ding, dingkan_at_simm.ac.cn)
Host glycosylation during bacterial infection	Deciphering the role of mononuclear phagocyte glycans in response to bacterial infection	Institute of Pharmacology and Structural Biology, CNRS-Toulouse University (Yoann Rombouts, yoann.rombouts_at_ipbs.fr)
Cell specific glycomics and glycoproteomics in autoimmunity and cancer	To characterise in a tissue and cell-specific manner (immune and non-immune cells) the glycans signature and its functional relevance in cancer and autoimmunity.	Institute of Molecular Pathology and Immunology of University of Porto (Salomé Pinho, salomep_at_ipatimup.pt)
Functional glycomics and glycoproteomics in cancer	To determine glycans functional modulation in cancer biology and therapy response.	Institute of Molecular Pathology and Immunology of the University of Porto (Celso Reis, celsor_at_ipatimup.pt)
The uromoduline glycome	Staging uromoduline glycosylation in controls and patients suffering from urogenital infections and kidney stones	Hannover Medical School (Rita Gerardy-Schahn, Gerardy-Schahn.Rita_at_mh-hannover.de)
Human milk oligosaccharide (HMO) biosynthesis and function	To study the biosynthetic pathway as well as structure function relationship of human milk oligosaccharides for infant health and development and throughout the lifecycle	UC San Diego Larsson- Rosenquist Foundation Mother- Milk-Infant Center of Research Excellence (Lars Bode)

5) Analytical methods and standards for glycoscience

Short Name	Description	Coordinating Institution
MS methods for high-throughput glycoproteomics	To develop and standardize analytical methods for high-throughput glycoproteomics	Leiden University Medical Centre (Manfred Wuhrer, m.wuhrer_at_lumc.nl)
Standards for glycomics and glycoproteomics	To synthesize, isolate, characterize and make available glycan and glycopeptide standards	Harvard Medical School (Richard Cummings, rcummin1_at_bidmc.harvard.edu)
Standards for glycoproteomics	To produce defined N-glycopeptides for glycoproteomics research	Institute for Glycomics, Griffith University (Daniel Kolarich, d.kolarich_at_griffith.edu.au)
De novo sequencing of carbohydrates	Hyphenated MS methods for de novo sequencing of carbohydrates	University of Manchester (Sabine Flitsch, Sabine.Flitsch_at_manchester.ac.uk)
Bioinformatic tools for glycoscience	To develop bioinformatic tools to generate, store, analyse and visualise glycan data	Swiss Institute for Bioinformatics (Frederique Lisacek, Frederique.Lisacek_at_sib.swiss)
UniCarb-DB	Curated database for access to searchable curated spectral glycoMS libraries (http://www.unicarb-db.org)	University of Gothenburg (Niclas Karlsson, niclas.karlsson_at_medkem.gu.se)
UniCarb-DR	Data repositories for supporting open access storage of glycan MS data from the glycocommunity based on best practice (http://unicarb-dr.biomedicine.gu.se/)	University of Gothenburg (Niclas Karlsson, niclas.karlsson_at_medkem.gu.se)
The Glycosmos Portal	GlyCosmos Portal is a portal site of glycoscience that aims to integrate life science data	Soka University (Kiyoko F. Aoki-Kinoshita, kkiyoko_at_soka.ac.jp)
Genetically encoded technologies for glycoscience	Genetically encoded technologies for spatio-temporal mapping of glycans and glycan binding proteins in cells and tissues in vivo	University of Alberta (Prof. Ratmir Derda, ratmir_at_ualberta.ca)
Molecular sensor of IgG glycosylation	Generation of simplified tools to probe antigen-specific IgG glycosylation	Sanquin Research/University of Amsterdam (Gestur Vidarsson, G.Vidarsson_at_sanquin.nl)
Analysis of glycosphingolipid-derived glycans by CGE-LIF	To develop tools for high-throughput glycosphingolipid analysis	Hannover Medical School (Falk Buettner, Buettner.Falk_at_mh-hannover.de)
Glycoengineering of cells	Glycan active enzymes as tools for glycoengineered cells	Hannover Medical School (Rita Gerardy-Schahn, Gerardy-Schahn.Rita_at_mh-hannover.de)

To include your projects in projects of the Human Glycome Project, please send short description of the project to the Steering Committee of the Human Glycome Project at info@human-glycome.org